Fig. 4
From: Oncogenic Ras is downregulated by ARHI and induces autophagy by Ras/AKT/mTOR pathway in glioblastoma
CQ can enhance the accumulation of autophagic vacuoles induced by ARHI in glioma cells. a The distribution of LN229 mRFP-GFP-LC3 immunofluorescence in cells transfected with ARHI and negative control plasmids cultured with 2 mM 3-MA or 3 μM CQ after 48 h was analyzed by fluorescence microscopy. Scale bar represents 30 μM. b The mRFP-GFP-LC3 expression levels in LN229 cells (the bar whiskers represent SD, *p < 0.05, **p < 0.01, ***p < 0.001). c TEM photomicrographs of LN229 cells. AVs, autophagic vacuoles; AVi, initial AVs (yellow arrows); AVd, late AVs (red arrows). The scale bar represents 2 μm. d Fluorescence microscopy of TUNEL expression in LN229 cells. The scale bar represents 100 μm