Fig. 2
From: Oncogenic Ras is downregulated by ARHI and induces autophagy by Ras/AKT/mTOR pathway in glioblastoma
Over-expression of ARHI can induce autophagy in glioma cells. a-b Representative transmission electron microscopy of LN229 and T98G cells after transfection with ARHI and negative control plasmids. The scale bar represents 2 μm. c-d Fluorescence microscopy of LC3B expression after over-expressing ARHI and negative control plasmids (yellow arrows indicate LC3B puncta). The scale bar represents 50 μm. e-f After transfecting LN229 and T98G cells with ARHI and negative control plasmids, ARHI, SQSTM1 and LC3B expression was examined by western blot assay