Skip to main content

Advertisement

Fig. 3 | BMC Cancer

Fig. 3

From: Differences in colonic crypt morphology of spontaneous and colitis-associated murine models via second harmonic generation imaging to quantify colon cancer development

Fig. 3

Summary of quantitative image features. Each image feature is described with an equation and a visual representation of the calculation using an SHG image of normal epithelium; scale bar is 100 μm. For eccentricity, a represents half the length of the major axis, and b represents half the length of the minor axis. For nearest neighbor, (xc,yc) represents the centroid coordinate and (xa,ya) represent the centroid coordinates of adjacent crypts; the arrows represent the distance to adjacent crypts, the red arrow represents the distance selected as the nearest neighbor. For the centroid distance functions (CDF), (xc,yc) represents the centroid coordinate and (xb,yb) represent the boundary pixel coordinates. The average CDF was calculated by averaging all the distances to the boundary pixels (red arrows); the minimum CDF was calculated by selecting the distance to the closest boundary pixel (red arrow); the maximum CDF was calculated by selecting the distance to the furthest boundary pixel (red arrow)

Back to article page