Fig. 6

JS-K induced autophagy-related protein and autophagosome changes in ovarian cancer A2780 and SKOV3 cells. a Autophagy-related proteins expression were measured using WB assay. b The quality graphs of the A. c GFP - LC3 plasmid were transfected to the two cell lines, confocal microscopy was used to observe the distribution. d TEM was used to observe intracellular morphology (24 h treatment). e WB method was used to examine LC3B I/II and p62 proteins expression in SKOV3 cells treated with JS-K/NAC. f MTT method analyzed the cell viability of SKOV3 cells co-treated with 3-MA and BAF for 48 h. g WB method was used to examine LC3B I/II distribution, p62, Bcl2 and Bax proteins expression in SKOV3 cells treated with JS-K/BAF. h WB method analyzed ATG5 SiRNA1^# decreased ATG5 protein expression. i Microscope observed the cell morphology and MTT method analyzed the cell viability of SKOV3 cells treated with JS-K/ATG5 SiRNA1^# for 48 h. —represents 40 μm