Fig. 5

NAC rescues JS-K-mediated cell death of A2780 and SKOV3. a The cell viability of different NAC concentration reversed JS-K-treated A2780 and SKOV3 cells were analyzed with MTT method. b JS-K and NAC co-treated cells were detected with microscope (200 × magnification). c Annexin V/PI staining of A2780 and SKOV3 cells apoptosis treated with 2.5 μM JS-K /NAC 200 μM (Mean ± SD, 48 h and 24 h treatment). d The Caspase family proteins 3/7/8/9 activity in A2780 and SKOV3 cells treated with 2.5 μM JS-K or 200 μM NAC as indication. (48 h treatment) were measured using ELISA assay. e The expression of apoptosis-related proteins in A2780 and SKOV3 cells treated with 2.5 μM JS-K /200 μM NAC (48 h treatment) were measured using WB assay. g Colony formation assay was used to detect cell proliferation of JS-K and NAC co-treated A2780 and SKOV3 cells (48 h treatment). —represents 40 μm. (f and h) The quality graphs of the e and g