Fig. 2

RT-PCR analysis of PCDH10 in human cancer cell lines. dHPLC analysis of RT-PCR amplified cDNA shows a peak indicating the expression of PCDH10 in the breast adenocarcinoma MB-231 cells, in line with the lack of methylation of the corresponding promoter in the same cells. Conversely, no peaks are detected in Capan-2 and AsPC-1 pancreatic adenocarcinoma and in AGS gastric adenocarcinoma cells, indicating lack of PCDH10 mRNA expression in these cell lines, whose PCDH10 promoter is fully methylated