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Fig. 3 | BMC Cancer

Fig. 3

From: Ascites from ovarian cancer patients stimulates MUC16 mucin expression and secretion in human peritoneal mesothelial cells through an Akt-dependent pathway

Fig. 3

Dose-dependent release of MUC16 in HPMCs treated with ascites (0,001–10%). a Meso7 cells were incubated with increasing concentrations of OVC508 or OVC690 ascites overnight, cells were rinsed 3 times and MUC16 shedding was determined in the conditioned medium 24 h later. b Meso7 cells were incubated overnight with ascites (10% v/v), rinsed 3 times and MUC16 shedding was determined after 24 h and up to 96 h post treatment. Levels were standardized according to the total protein concentration. Results are from two independent experiments performed in duplicates

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