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Fig. 1 | BMC Cancer

Fig. 1

From: Ascites from ovarian cancer patients stimulates MUC16 mucin expression and secretion in human peritoneal mesothelial cells through an Akt-dependent pathway

Fig. 1

Expression of MUC16 in HPMCs. a Immunohistochemistry staining for MUC16 on two different HPMCs samples (Meso 4 and Meso 7) grown on glass slides using anti-CA125 M11 antibody (left panel). HPMC Meso 7 were cultured on a glass slide and stained with anti-αSMA antibody (right panel). Representative images are shown. b Immunofluorescence detection of MUC16, calretinin and αSMA in Meso7 HPMCs. The cells were fixed, permeabilized and incubated with anti-CA125 M11, anti-calretinin or anti-αSMA antibodies. HPMCs stained positive for calretinin and αSMA but negative for MUC16 confirming their mesenchymal phenotype. Representative images are shown. c Expression of MUC16 in Meso7 HPMCs and ovarian cancer cell line OVCAR3 was determined by immunoblot using anti-CA125 M11 antibody. Tubulin was used as a loading control. The ovarian cancer cell line OVCAR3 was used as a positive control for MUC16 expression

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