Table 1 Docetaxel progressively reduces the numbers of external SK-MEL-28. Tri-culture spheroids were harvested after 15, 24, 48, and 72 h of treatment with 100 nM of docetaxel or 0.01 ‰ of DMSO. HaCaT and SK-MEL-28 cells were labeled with CellTrackerRed CMPTX and CellTrackerGreen CMFDA dyes, respectively. Upon cryosectioning into 10-μm thick slices, labeling of nuclei with Dapi, and confocal imaging, external SK-MEL-28 cells were counted using ImageJ. Shown is mean ± SEM of the number of external SK-MEL-28 cells over time (n ≥ 3 independent experiments)

From: A novel spheroid-based co-culture model mimics loss of keratinocyte differentiation, melanoma cell invasion, and drug-induced selection of ABCB5-expressing cells