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Table 1 Docetaxel progressively reduces the numbers of external SK-MEL-28. Tri-culture spheroids were harvested after 15, 24, 48, and 72 h of treatment with 100 nM of docetaxel or 0.01 ‰ of DMSO. HaCaT and SK-MEL-28 cells were labeled with CellTrackerRed CMPTX and CellTrackerGreen CMFDA dyes, respectively. Upon cryosectioning into 10-μm thick slices, labeling of nuclei with Dapi, and confocal imaging, external SK-MEL-28 cells were counted using ImageJ. Shown is mean ± SEM of the number of external SK-MEL-28 cells over time (n ≥ 3 independent experiments)

From: A novel spheroid-based co-culture model mimics loss of keratinocyte differentiation, melanoma cell invasion, and drug-induced selection of ABCB5-expressing cells

  DMSO control 100 nM docetaxel
15 h 80.4 ± 6.6 81.1 ± 4.0
24 h 77.6 ± 6.9 58.4 ± 6.2 **
48 h 142.9 ± 20.5 33.1 ± 3.1 **
72 h 135.2 ± 1.9 19.7 ± 3.2 **