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Fig. 1 | BMC Cancer

Fig. 1

From: A novel spheroid-based co-culture model mimics loss of keratinocyte differentiation, melanoma cell invasion, and drug-induced selection of ABCB5-expressing cells

Fig. 1

Proliferation, apoptosis, and differentiation of fibroblasts and HaCaT cells in mono- and bi-culture spheroids. Spheroids were cultured as mono- and bi-cultures for seven days, cryosectioned into 10-μm thick slices, and then stained for markers for cell proliferation (a, Ki67, green), apoptosis (b, CAS3, green), differentiated (c, CK10, green) and basal keratinocytes (c, CK14, red). In a and b, nuclei were stained with Dapi (blue). a-c Representative confocal images. Scale bars: 100 μm. d Quantification of Ki67- and CAS3-positive cells (percentage of total) and CK10-positive cells (percentage of peripheral nuclei). Given is mean ± SEM (n ≥ 3 independent experiments; ** P < 0.01)

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