Fig. 2

Numb overexpression inhibits proliferation, invasion and migration of SCC-9 and CAL-27 cells. a Cell proliferation assay and quantification of NC (mock) or Numb-overexpressed SCC-9 and CAL-27 cells by MTT assay at 48 h after transfection. NC denotes cells transfected with empty vector, Numb denotes cells transfected with pCDH-Numb. b and c Wound healing assay and quantification shows the migration ability of NC (mock) or Numb-overexpressed SCC-9 and CAL-27 cells. The photographies were taken at the time points of 0, 24, 48 h. Magnification × 10(5000 μm) in wound healing assay. d and e Transwell assay and quantification shows the invasion ability of NC (mock) or Numb-overexpressed SCC-9 and CAL-27 cells. The photographies were taken at 24 h. Magnification× 50(2000 μm) in transwell assay. The data were represented as mean ± SD from 3 biological replicates (*p < 0.05; **p < 0.01; ***p < 0.001)