Fig. 7

Up- or downregulation of hsa_circ_0005379 alters the expression levels of key proteins involved in cell proliferation, apoptosis, invasion, and the EGFR signaling pathway. a, c SCC25 and CAL27 cells were subjected to either overexpression (a) or knockdown (c) of hsa_circ_0005379 and cell extracts were immunoblotted with the indicated antibodies. b Key EMT protein levels were detected by western blotting after upregualtion of hsa_circ_0005379 in SCC25 and CAL27 cells. d, e EGFR and p-EGFR protein levels were detected by western blotting after down- (d) or upregulation (e) of hsa_circ_0005379. f The SCC25 and CAL27 cells were treated with EGFR inhibitor cetuximab or agonist NSC. The expression levels of hsa_circ_0005379 were quantified by qRT-PCR. Data are presented as means ± SEM of three independent experiments. Student’s t-test, n.s., not significant