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Fig. 4 | BMC Cancer

Fig. 4

From: Salinomycin triggers endoplasmic reticulum stress through ATP2A3 upregulation in PC-3 cells

Fig. 4

Salinomycin-triggered ER stress might be related to ATP2A3-mediated Ca2+ release. a. Flow cytometry showed that salinomycin inhibited Ca2+ release in PC-3 and DU145 cells. b. Images were captured using an inverted fluorescence microscope after salinomycin treatment. Scale bars, 10 μm. c. ATP2A3 siRNAs (#1, #2 and #3) were respectively transfected in PC-3 cells for 48 h. Western blotting analyzed ATP2A3 protein expression. d. ATP2A3 silencing weakened salinomycin-triggered ER stress in PC-3 cells. e. ATP2A3 silencing reduced salinomycin-inhibited Ca2+ release in PC-3 cells. f. Salinomycin changed CaMK activity. The CaMK-II protein expression level and phosphorylation level (Thr 286) were analyzed by Western blotting. g. Western blotting showed that BAPTA-AM (10 μM, an intracellular calcium chelator) weakened salinomycin-triggered ER stress

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