Fig. 2

Mifepristone attenuates migration and invasion of cancer cells of the ovary (a, e), breast (b, f), prostate (c, g), or glia (d, h) through filter-based assays assessed by phase contrast microscopy. Cells were exposed to either vehicle of cytostatic concentrations of MF specific to each cell line for 72 h and then seeded into Boyden chambers. At various time-points (9, 18, and 24 h) cells that migrated through the insert were counted after staining with crystal violet. Data shown represent the mean ± s.e.m. of three independent experiments completed in triplicate (a-d). Vehicle (closed bars) and MF (open bars). Images at the right of panel A show inserts of SKOV-3 cells treated with vehicle or MF, 24 h following migration; white arrows: cells that migrated through the pores and re-attached to the other side of the membrane; yellow arrowheads: 8 μm membrane pores. Scale bars = 50 μm. *P < 0.05, **P < 0.01and ***P < 0.001 compared against vehicle. Statistical analysis was done using a two-way ANOVA followed by Bonferroni’s multiple-comparison test. For the invasion assays, data shown represent the mean ± s.e.m. of three independent experiments completed in triplicate. Vehicle (closed bars); MF (open bars)