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Fig. 2 | BMC Cancer

Fig. 2

From: HPV DNA integration site as proof of the origin of ovarian metastasis from endocervical adenocarcinoma: three case reports

Fig. 2

Identification of the HPV integration site in 2q22.3 in both cervical and ovarian tumors, case 2. a The junction between HPV18 (grey box) and the human genome is indicated by a vertical grey line. The coordinate of the human genome breakpoint (146477121) in the cervical tumor was determined according to the forward strand (+) of the hg19 reference. Horizontal arrows indicate the sites of the primers designed to specifically amplify the human/viral junction sequence. The genes closest to the HPV integration site are identified at 643 kb (TEX41) and 2 Mb (ACVR2) and shown as black boxes. b Analysis of the PCR products on agarose gel. Lane 1 represents molecular weight, lanes 2 and 3 indicate cervical and ovarian tumor DNA, respectively. Lane 4 is DNA from another patient (negative control). c Sanger sequencing chromatogram obtained from cervical tumor DNA (upper part) and ovarian tumor DNA (lower part). The vertical grey line indicates the junction between HPV18 (left part) and the human genome (right part)

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