Fig. 5

MNRR1 upstream of known metastasis-associated genes. a List of genes identified by RNA-Seq to be significantly downregulated in the HEK293 R1-KO cells and rescued in the R1-KO cells expressing the transcriptionally active version of MNRR1 (R1-TA). The PubMed IDs (PMID) of the studies published indicating the role of each of the genes in cancer metastasis is shown. b AKT3 (circled) is the most upregulated gene in the MNRR1-knockout cells. c Total AKT3 protein levels in WT, R1-KO, and R1-OE cells with Actin used as a loading control. d MCF7 cells (WT) with a knockout of MNRR1 (MCF7 R1-KOm; KO) also display an increase in total AKT3 protein levels. GAPDH is used as a loading control