Table 1 Primers used for 16S rRNA gene sequencing and qPCR analysis

Bacteria and Archaea (sequencing)

V4 variable region of the 16S rRNA (515F and 806R [51])

F: 5′-GTGCCAGCMGCCGCGGTAA-3′

R: 5′-GGACTACHVGGGTWTCTAAT-3´

65.4

49.0

~ 250–390 bp^a

S. gallolyticus spp.

SodA

(AP012053)

(HE613569)

(AP012054)

F: 5´-GCTTGGCTTGTGGTGAATGA-3′

R: 5′-GCGAACGTTGCGATACTTGA-3´

59.0

59.3

144

S. gallolyticus subsp. gallolyticus

SodA (AP012053)

F: 5´- AAGCTGCGACAACTCGCTTT − 3′

R: 5′- AAGCGTGTTCCCAAACGTCA − 3´

61.1

60.8

150

F. nucleatum

16 s ribosomal RNA (CP012717)

F: 5´–CCCAAGCAAACGCGATAAGT–3′

R: 5´–GCGTTGCGTCGAATTAAACC–3´

59.2

58.9

117

B. fragilis

16 s ribosomal RNA (M11656)

F: 5′- AGTAGAGGTGGGCGGAATTC − 3′

R: 5′- GTGTCAGTTGCAGTCCAGTG − 3´

59.2

59.1

97

β-actin

β-actin (NG_007992)

F: 5´-ACTCGTCATACTCCTGCTTGC-3′

R: 5′-CCTCCTCAGATCATTGCTCCTC-3´

60.1

60.0

118