Fig. 6

GC-mediated protection against TNF is diminished by inhibiting c-IAP1 or XIAP expression. Western blot immunoassay of MCF7 cells transfected with the four siRNAs against c-IAP1 (a) or XIAP (b) for 18 h. MOCK: no siRNA; NR-siRNA: nonrelated siRNA; c-IAP1-siRNA and XIAP-siRNA, from 1 through 4 (with four different sequences each). The histograms present the ratios of normalized siRNA-targeted c-IAP1 and XIAP expression to normalized β-actin expression. For transfections, we used the lowest IAP/β-actin ratio. Vertical lines indicate the standard deviation of the mean (*p < 0.005). Data are presented as the mean ± standard deviation [SD]; n = 3. c) Viability assay of MCF7 cells treated for 24 h with the indicated treatments. An average of two independent experiments was performed with each sample conducted in sextuplicate. Vertical lines represent the standard deviation of the mean; *p < 0.05. Letters indicate distinct groups based on the post hoc statistical comparison (p < 0.05). If groups share at least one letter, they do not statistically differ. Data are presented as the mean ± SD; n = 2