Fig. 5

Cortisol and dexamethasone mediate sustained protein levels of c-IAP1 and XIAP during protection from TNF. Western blot immunoassay detecting c-IAP1 a or XIAP c using 30 μg of total proteins from MCF7 cells a and c treated with ethanol (ETOH), cortisol (CORT), or CORT + TNF for different lengths of time (3, 6, 12, 18, 24, 36, and 48 h). Lane 1 corresponds to cells treated with only vehicle (ETOH) for the indicated time b and d. Histograms representing panel a and panel c with the ratios of normalized c-IAP1 (panel a) and XIAP (panel c) levels to the β-actin signal (relative density [RD]) in cells treated with CORT. Vertical lines indicate the standard deviation (SD) of the mean (*p < 0.005). Data are presented as the mean ± SD; n = 3. Panels e and g are the same as a and c but with DEX instead of CORT. Panels f and g are the same as b and d, but with DEX instead of CORT. (*p < 0.005). Data are presented as the mean ± SD; n = 3