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Fig. 4 | BMC Cancer

Fig. 4

From: Depletion of PCAT-1 in head and neck cancer cells inhibits tumor growth and induces apoptosis by modulating c-Myc-AKT1-p38 MAPK signalling pathways

Fig. 4

Depletion of PCAT-1 activates p38 MAPK through ASK1 and induces Caspase mediated apoptosis in HNSCC. Protein lysates from JHU029 and Cal27 cells transfected with control or siRNA to PCAT-1 were subjected to Western blot analysis using specific antibodies. a: Expression of phospho-ASK1 and total ASK1 and the right panel shows quantitation of the data using Image J Software b: Expression of phospho-p38 and total p38 and right panel shows quantitation of the data. The membranes were reprobed with antibody to GAPDH as an internal control. Small bar indicates standard error (*, p < 0.05; ** p < 0.01). c: Western blot analysis of the cell lysates was performed using the specific antibodies to PARP or Caspase 9. The membrane was reprobed with antibody to GAPDH as an internal control

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