Fig. 1From: Depletion of PCAT-1 in head and neck cancer cells inhibits tumor growth and induces apoptosis by modulating c-Myc-AKT1-p38 MAPK signalling pathwaysPCAT-1 expression is enhanced in HNSCC and regulates cell proliferation. a: PCAT-1 expression in HNSCC patient samples compared to the non-tumor tissues from the TCGA data. P value <â0.05 was selected as cut off. b: Relative mRNA expression of PCAT-1 gene in HNSCC patient samples (nâ=â23) compared to adjacent non-tumor tissues. 18âs gene used as internal control. c: Relative mRNA expression of PCAT-1 in HNSCC cell lines compared to NOK. 18âs gene used as internal control. d: JHU029 and Cal27 cells were transfected with PCAT-1 siRNA (50ânM) and after 48âh, PCAT-1 knockdown efficiency was checked by analysing mRNA expression. 18âs gene was used as internal control. e: JHU029 and Cal27 cells were transfected with/ without siRNA. At 0âh, 48âh and 96âh cell were stained with trypan blue and percentage of live cells was counted using a haemocytometer. Small bar indicates standard error (*, pâ<â0.05; ** pâ<â0.01; ***pâ<â0.001)Back to article page