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Fig. 1 | BMC Cancer

Fig. 1

From: Depletion of PCAT-1 in head and neck cancer cells inhibits tumor growth and induces apoptosis by modulating c-Myc-AKT1-p38 MAPK signalling pathways

Fig. 1

PCAT-1 expression is enhanced in HNSCC and regulates cell proliferation. a: PCAT-1 expression in HNSCC patient samples compared to the non-tumor tissues from the TCGA data. P value < 0.05 was selected as cut off. b: Relative mRNA expression of PCAT-1 gene in HNSCC patient samples (n = 23) compared to adjacent non-tumor tissues. 18 s gene used as internal control. c: Relative mRNA expression of PCAT-1 in HNSCC cell lines compared to NOK. 18 s gene used as internal control. d: JHU029 and Cal27 cells were transfected with PCAT-1 siRNA (50 nM) and after 48 h, PCAT-1 knockdown efficiency was checked by analysing mRNA expression. 18 s gene was used as internal control. e: JHU029 and Cal27 cells were transfected with/ without siRNA. At 0 h, 48 h and 96 h cell were stained with trypan blue and percentage of live cells was counted using a haemocytometer. Small bar indicates standard error (*, p < 0.05; ** p < 0.01; ***p < 0.001)

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