Skip to main content

Advertisement

Table 1 Nested-PCR amplification primers and protocols for EGFR exon 21

From: Intratumoral heterogeneity of EGFR-activating mutations in advanced NSCLC patients at the single-cell level

  Primers Sequence 5′-3′ PCR conditions PCR product (bp)
External PCR amplification External TCAGAGCCTGGCA 1) 95 °C, 5 min 297
Forward TGAACATGACCCTG 61 °C, 30 s
72 °C, 30 s
3) 72 °C, 7 min2)35 cycles:
   95 °C, 45 s
External GGTCCCTGGTGTC 61°C, 30 s
  Reverse AGGAAAATGCTGG 72°C, 30 s
3) 72°C, 7 min
Internal PCR amplification Internal ATGAACTACTTG 1) 95 °C, 5 min 188
Forward GAGGACCGT 2)35 cycles:
Internal GAAAATGCTGGCT 95 °C, 45 s
Reverse GACCTAAG 60 °C, 30 s
72 °C, 30 s
3)72 °C, 7 min