Table 1 Primer sequences used in this study
Gene | Sequence | ||
|---|---|---|---|
Nodal | Promoter | Forward | 5′-ACAGCCTCCTCCCCTAAGACAGA-3’ |
Reverse | 5′-GAAAGCAGCACCTCCAGCCCTTA-3’ | ||
cDNA | Forward | 5′-ACCATGCACGCCCACTGCCTG-3’ | |
Reverse | 5′-ATGTCATCAGAGGCACCCACA-3’ | ||
mRNA | Forward | 5′-AGACATCATCCGCAGCCTAC-3’ | |
Reverse | 5′-GTCCATCTGAAACCGCTCTA-3’ | ||
Mut-2TA | Forward | 5′-GTGCTCCTTATGCTCTACTCCAACCTCTCG-3’ | |
Reverse | 5′-ATTGGCGGCAGGCGGTGCGG-3’ | ||
TGF-β1 | mRNA | Forward | 5′-TACTACGCCAAGGAGGTCAC-3’ |
Reverse | 5′-ATTTCCCCTCCACGGCTCAA-3’ | ||
ISH | Forward | 5′-AGTTGTGCGGCAGTGGTTGA-3’ | |
Reverse | 5′-CGGGACCTCAGCTGCACTT-3’ |