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Fig. 4 | BMC Cancer

Fig. 4

From: Reduced FRG1 expression promotes prostate cancer progression and affects prostate cancer cell migration and invasion

Fig. 4

Effect of FRG1 expression on LNCaP cell properties. a. Western blot to confirm ectopic expression of FRG1 in LNCaP cells. Western blot to confirm depletion of FRG1 expression in LNCaP cells after RNAi silencing. b. Graphical representation of LNCaP cell proliferation assay showing that ectopic expression of FRG1 has no significant (N = 5, 2-tailed unpaired t-test, p value > 0.05) effect on cell proliferation, compared to empty vector control (pCMV6.XL5). c. Graph showing that knockdown of FRG1 (FRG1 KD) doesn’t change LNCaP cells proliferation significantly (N = 5, 2-tailed unpaired t-test, p value > 0.05), compared to control (pLKO.1sc). d. Representative images of transwell migration assay of LNCaP cells with ectopic expression of FRG1 (FRG1) and respective vector control (pCMV6.XL5). e. Graphical representation of transwell migration assay of LNCaP cells with ectopic expression of FRG1 (601 ± 54), showing no change in transwell migration, compared to empty vector control (622 ± 71) (N = 3, 2-tailed unpaired t-test, p value > 0.05). f. Representative images of transwell migration assay of LNCaP cells with FRG1 knockdown (FRG1 KD) and respective scrambled vector control (pLKO.1sc). g. Graph of transwell migration assay of LNCaP cells with FRG1 knockdown (645 ± 61), showing no change in transwell migration, compared to scrambled vector control (550 ± 55) (N = 3, 2-tailed unpaired t-test, p value > 0.05). h. Representative images of matrigel invasion assay of LNCaP cells with ectopic expression of FRG1 and respective vector control. i. Graphical representation of matrigel invasion assay of LNCaP cells with ectopic expression of FRG1 (483 ± 130) compared to empty vector control (484 ± 89) (N = 3, 2-tailed unpaired t-test, p value > 0.05). j. Representative images of matrigel invasion assay of LNCaP cells with FRG1 knockdown (FRG1 KD) and respective scrambled vector control (pLKO.1sc). k. Representative graph of matrigel invasion assay of LNCaP cells with FRG1 knockdown (666 ± 60) compared to scrambled vector control (589 ± 24) (N = 3, 2-tailed unpaired t-test, p value > 0.05). # represents p value > 0.05, N represents experiment replicates

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