Fig. 3
Effect of FRG1 expression on transwell migration and invasion: a. Representative images of transwell migration assay of DU145 cells with ectopic expression of FRG1 and respective vector control. b. Graphical representation of transwell migration assay of DU145 cells with ectopic expression of FRG1 (2183 ± 84), showing reduction in transwell migration, compared to empty vector control (2565 ± 94) (N = 3, 2-tailed unpaired t-test, p value < 0.01). c. Representative images of transwell migration assay of DU145 cells with FRG1 knockdown and respective scrambled vector control. d. Graph of transwell migration assay of DU145 cells with FRG1 knockdown (1532 ± 165), showing enhanced transwell migration, compared to scrambled vector control (987 ± 130) (N = 3, 2-tailed unpaired t-test, p value < 0.05). e. Representative images of transwell migration assay of PC3 cells with ectopic expression of FRG1 and respective vector control. f. Graphical representation of transwell migration assay of PC3 with ectopic expression of FRG1 (1869 ± 86), showing reduction (N = 3, 2-tailed unpaired t-test, p value < 0.005) in transwell migration, compared to empty vector control (2256 ± 81). g. Representative images of transwell migration assay of PC3 cells with FRG1 knockdown and respective scrambled vector control. h. Graphical representation of transwell migration assay of PC3 cells with FRG1 knockdown (1685 ± 120), showing enhanced (N = 3, 2-tailed unpaired t-test, p value < 0.01) transwell migration, compared to scrambled vector (1285 ± 71). i. Representative images of matrigel invasion assay of DU145 cells with ectopic expression of FRG1 and respective vector control. j. Graphical representation of matrigel invasion assay of DU145 cells with ectopic expression of FRG1 (231 ± 43) compared to empty vector control (357 ± 60) (N = 3, 2-tailed unpaired t-test, p value < 0.05). k. Representative images of matrigel invasion assay of DU145 with FRG1 knockdown and respective scrambled vector control. l. Representative graph of matrigel invasion assay of DU145 cells with FRG1 knockdown (412 ± 62) compared to scrambled vector control (234 ± 27) (N = 3, 2-tailed unpaired t-test, p value < 0.05). m. Representative images of matrigel invasion assay of PC3 cells with ectopic expression of FRG1 and respective vector control. n. Representative graph of matrigel invasion assay of PC3 cells with ectopic expression of FRG1 (208 ± 65) compared to empty vector control (365 ± 53) (N = 3, 2-tailed unpaired t-test, p value < 0.05). o. Representative images of matrigel invasion assay of PC3 with FRG1 knockdown and respective scrambled vector control. p. Representative graph of matrigel invasion assay of PC3 cells with FRG1 knockdown (318 ± 29) compared to scrambled vector control (207 ± 37) (N = 3, 2-tailed unpaired t-test, p value < 0.05). * represents p value ≤0.05, ** represents p value < 0.01, *** represents p value < 0.005, N represents experiment replicates