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Fig. 2 | BMC Cancer

Fig. 2

From: Reduced FRG1 expression promotes prostate cancer progression and affects prostate cancer cell migration and invasion

Fig. 2

Effect of FRG1 expression on cell proliferation and scratch wound healing: a. Ectopic expression of FRG1 has no significant (N = 5, 2-tailed unpaired t-test, p value > 0.05) effect on cell proliferation in DU145, compared to empty vector control. b. Measurement of cell proliferation in DU145, knockdown of FRG1 showing significant increase in cell proliferation (N = 5, 2-tailed unpaired t-test, p value < 0.05), compared to scrambled vector control. c. Measurement of cell proliferation in PC3 with ectopic expression of FRG1, compared to empty vector control, showing significant reduction (N = 5, 2-tailed unpaired t-test, p value < 0.05). d. Quantitation of cell proliferation in PC3 with knockdown of FRG1, compared to scrambled vector control, showing no significant (N = 5, 2-tailed unpaired t-test, p value > 0.05) effect. e. Representative images of scratch wound healing assay of DU145 cells, with ectopic expression of FRG1 and respective vector control. f. Graph of scratch wound healing assay of DU145 cells with ectopic expression of FRG1, showing 52% reduced wound area, compared to empty vector control with 74% reduced wound area (N = 3, 2-tailed unpaired t-test, p value < 0.05). g. Representative images of scratch wound healing assay of DU145 with FRG1 knockdown and respective scrambled vector control. h. Graph of scratch wound healing assay of DU145 cells with FRG1 knockdown showing 91% reduced wound area, compared to scrambled vector control with 68% reduced wound area (N = 3, 2-tailed unpaired t-test, p value < 0.05). i. Representative images of scratch wound healing assay of PC3 cells with ectopic expression of FRG1 and respective vector control. j. Graph of scratch wound healing assay of PC3 cells with ectopic expression of FRG1, showing 57% reduced wound area, compared to empty vector control with 90% reduced wound are (N = 3, 2-tailed unpaired t-test, p value < 0.05). k. Representative images of scratch wound healing assay of PC3 with FRG1 knockdown and respective scrambled vector control. l. Graph of scratch wound healing assay of PC3 cells with FRG1 knockdown, showing 80% reduced wound area, compared to scrambled vector control with 70% reduced wound area (N = 3, 2-tailed unpaired t-test, p value < 0.005). # represents p value > 0.05, * represents p value ≤0.05, ** represents p value < 0.01, *** represents p value < 0.005, N represents experiment replicates

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