Fig. 1

FRG1 expression levels in prostate tumor and cell lines: a. Representative images of tumor and uninvolved tissues of prostate, as seen in first (uninvolved) and second (tumor) column from left. b. Comparison of IRS between tumor and uninvolved tissue. Graph shows that the reduction of IRS in tumor tissue (N = 100) compared to uninvolved adjacent tissue (N = 100) was significant (chi square test, 2 tailed, df-5, p value < 0.0005). Median IRS score for FRG1 in tumor is 2.5 compared to adjacent uninvolved tissue, which is 3.5. c. Distribution of staining pattern for FRG1 in the prostate tumor (N = 100) and uninvolved tissue (N = 100). d. FRG1 expression levels in three different prostate cancer cells, western blot panel shows comparatively higher levels of FRG1 in PC3 and LNCaP than DU145 cells. e. Western blot to confirm ectopic expression of FRG1 in DU145 cells. f. Western blot showing reduced FRG1 levels after RNAi silencing in DU145. g. Western blot image validating ectopic expression of FRG1 levels in PC3 cells. h. Reduction in FRG1 levels in FRG1 silenced PC3 cells confirmed by western blot. **** represents p value < 0.0005, N represents number of patient samples