Fig. 3

LAPTM4B promotes the proliferation, migration and invasion of LAC cells. a Western blotting analysis of LAPTM4B expression in human bronchial epithelial BEAS-2B cells and five LAC cell lines. β-actin was used as a loading control. b The protein levels were measured by Image J software. The expression level of LAPTM4B in BEAS-2B was set to 1.0. c Cells were infected with LAPTM4B overexpression lentivirus in A549 cells and transfected with specific LAPTM4B siRNAs in HCC827 cells. Endogenous LAPTM4B expression was indicated by the bottom band (35kDA) and the top band (38kDA) represented the exogenous LAPTM4B overexpression. Relative LAPTM4B protein levels were measured by Image J. d In CCK-8 assays, overexpression of LAPTM4B significantly increased the growth rate of A549, while downregulation of endogenous LAPTM4B significantly reduced the growth rate of HCC827 cells. Each bar represents the mean values±SD of three independent experiments. e Overexpression of LAPTM4B increased, while downregulation of endogenous LAPTM4B reduced, the colony numbers in colony formation assay. Each bar represents the mean values±SD of three independent experiments. f and g Overexpression of LAPTM4B increased, while downregulation of LAPTM4B reduced, the migration ability (f) and invasion ability (g) of A549 and HCC827 cells. Each bar represents the mean values±SD of three independent experiments. **P < 0.01, ***P < 0.001