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Fig. 6 | BMC Cancer

Fig. 6

From: Elucidating the transcriptional program of feline injection-site sarcoma using a cross-species mRNA-sequencing approach

Fig. 6

Cross-species (feline, canine, and human) analysis of soft-tissue sarcoma transcriptomes shows significant overlap. a Species-specific differential expression ratios (sarcoma/normal) of 53 genes that were selected because they are upregulated in sarcoma vs. normal tissue in three species: FISS, human soft tissue sarcoma, and canine soft tissue sarcoma. Genes are grouped vertically by gene annotation (curly braces). ECM, extracellular matrix. Gene symbol mappings to stable gene identifiers are provided in Additional file 3: Table S2 online. Gene symbols highlighted in red indicate genes whose upregulation in FISS was validated at the protein level. b Species-specific differential expression ratios (sarcoma/normal) of 47 genes that have mixed differential expression (i.e., not all upregulated and not all downregulated) among the feline, canine, and human sarcoma transcriptome profiling studies. c Species-specific differential expression ratios (sarcoma/normal) of 38 genes that are downregulated in sarcoma vs. normal tissue in FISS, human soft tissue sarcoma, and canine soft tissue sarcoma. Genes are grouped vertically by gene annotation (curly braces). d Cell-line-transcriptome-based bioinformatic screen for drug and drug-target enrichment among 53 genes that are upregulated in sarcoma in all three species. White bars correspond to pairs of drugs and cell lines (or drugs only, representing results from a screen against multiple cell lines), and gray bars correspond to drug targets. Bar height corresponds to the enrichment z score from Enrichr. Cell lines are indicated parenthetically. e Cell-line-transcriptome-based bioinformatic screen for drug and drug-target enrichment among 38 genes that are downregulated in sarcoma in all three species. Bar height indicates the Enrichr z score and bar fill color indicates whether the enrichment is for a drug or drug-target, as in panel (d). f Membrane image of immunoblot for protein BARX1 in lysates from fibroblasts (“cat01 fibrobl.”) and FISS-derived cell lines (“cat04 FISS cells” and “cat05 FISS cells”). The 27 kDa chain is BARX1 and the 40 kDa chain is a normalization control band from Memcode total protein stain (see Methods). Full membrane image is available as Additional file 7: Figure S2A–B. Quantified band intensities are provided in Additional file 6: Table S4. g Membrane image of immunoblot for protein FN1 in lysates from fibroblasts (“cat01 fibrobl.”) and FISS-derived cell lines (“cat05 FISS cells”). The 220 kDa chain is FN1 and the 40 kDa chain is a normalization control band from Memcode total protein stain (see Methods). Full membrane image is available as Additional file 7: Figure S2C–D. Quantified band intensities are provided in Additional file 8: Table S5

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