Fig. 1

a Comparison of mutation rates: Box-and-whisker plot showing the range, median, and 25th and 75th percentile levels of the number of mutations in all primary, pre-FOLFOX metastatic, and post-FOLFOX metastatic tumor samples. The difference in the number of mutations was not significant among the primary, pre-FOLFOX metastatic, and post-FOLFOX metastatic tumor samples across all cases (P > 0.05, Wilcoxon signed-rank test) b Comparison of mutation spectra: Stacked bar plot showing the proportion of mutations accounted for by each of the six mutation types in all primary, pre-FOLFOX metastatic, and post-FOLFOX metastatic tumor samples for each case