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Fig. 3 | BMC Cancer

Fig. 3

From: First-in-class immune-modulating small molecule Icaritin in advanced hepatocellular carcinoma: preliminary results of safety, durable survival and immune biomarkers

Fig. 3

Icaritin blocks the immune-suppression in myeloid cells in vitro. a. Icaritin treatment induced gene expression patterns of M1-type and M2-type macrophages in vitro. The relative copy numbers of M1-type genes (INOS, TNF-α and CXCL10) and M2-type genes (Arg1, Ym1 and Fizz1) were induced by icaritin (2 and 10 μM) and normalized to β-actin. b. Icaritin treatment down-regulates immune MDSCs (M-CSFR expression, a key marker of activated MDSCs). Flow cytometric analysis of M-CSFR in cytokine-induced CB-MDSCs with or without icaritin (2.5 μM, 72 h) treatment with CD14+ and CD15+ sorting. Summary of cell population ratio of CD14+M-CSFR+ and CD15+M-CSFR+ cells in cytokine-induced CB-MDSCs with or without icaritin treatment (*p < 0.05, see additional file for method details)

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BMC Cancer

ISSN: 1471-2407

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