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Fig. 3 | BMC Cancer

Fig. 3

From: A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform

Fig. 3

Measurement of lactate dehydrogenase (LDH) and dead-cell protease (DCP) release into culture effluent for the evaluation of cell membrane damage. a: Lactate dehydrogenase release from both malignant and benign thyroid tissue (n = 14 and n = 10, respectively unless otherwise stated; relative absorbance of formazan reaction product) during on-chip culture of thyroid tissue normalised per mg starting tissue wet weight. b: Level of relative light units (RLU) produced as a result of cleavage of the luminogenic assay substrate by dead cell protease released per mg of on-chip thyroid tissue over the 96 h culture period (n = 11). Lysis buffer (10% v/v) was added to homogenised tissue after 96 h culture (arrow) in order to purposefully rupture cell membranes and induce complete release of remaining LDH/ DCP

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