Table 1 Characteristics of antibodies
Artificial color | Clone | Provider | Concentration | Origin | Secondary antibody | |
|---|---|---|---|---|---|---|
Extracellular matrix-related fibroblast markers | ||||||
DAPI |
| Sigma-Aldrich | 2μg/ml | |||
PDGFR-β |
| 3169S | Cell Signaling | 1:50 | Rabbit | AlexaFluor 488 |
FAP |
| AF3715 | R&D Systems | 1:20 | Sheep | AlexaFluor 546 |
CD31 |
| JC70A | Dako | 1:1000 | Mouse IgG1 | AlexaFluor 594 |
α-SMA |
| 1A4 | Dako | 1:100 | Mouse IgG2a | AlexaFluor 680 |
Immune-related fibroblast markers | ||||||
DAPI |
| Sigma-Aldrich | 2μg/ml | |||
CD45 |
| PD7/2 + 2B11 | Dako | 1:1000 | Mouse IgG1 | AlexaFluor 514 |
FSP1 |
| D9F9F | Cell Signaling | 1:1000 | Rabbit | AlexaFluor 594 |
α-SMA |
| 1A4 | Dako | 1:100 | Mouse IgG2a | AlexaFluor 680 |
