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Fig. 2 | BMC Cancer

Fig. 2

From: Interaction between the BAG1S isoform and HSP70 mediates the stability of anti-apoptotic proteins and the survival of osteosarcoma cells expressing oncogenic MYC

Fig. 2

Individual expression of BAG1 isoforms revealed BAG1S critical to inhibit MYC-dependent cell death. a Schematic detailing the structure of human BAG1 isoforms L, M, and S. Amino acid positions shown on the top. Apparent molecular weights in kDa of protein products listed on the right. All isoforms share a ubiquitin-like domain, BAG domain, and complete (BAG1L and M) or partial (BAG1S) TESEEX hexapeptide repeats. Nuclear localization signal (NLS) domain present in BAG1L and partially in BAG1M. b U2OS MYC-ER cells transfected with vectors encoding distinct BAG1 isoforms L, M, S and an empty vector control. Cells depleted of endogenous BAG1 via infection with an shRNA-encoding lentivirus directed against the 3’UTR. IB demonstrating individual BAG1 isoform expression. c U2OS MYC-ER cells expressing distinct BAG1 isoforms depleted of endogenous BAG1 and treated with ±100 nM 4-OHT for 48 h. Apoptosis measured by Annexin V-PE plus 7AAD DNA staining and quantified by FACS analysis. d Quantification of three experimental replicates representing average and standard deviation of cumulative early and late apoptosis based on population of Annexin V-PE positive cells. e PARP cleavage and BAG1 expression demonstrated by IB from the same experiment but run on two separate gels. F and C indicate full and cleaved species respectively. *p < 0.05

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