Fig. 2

Key-proteins related to the mTOR pathway: phosphorylated Akt-PKB, Erk/MAPK, and IRS-1 in the gastrocnemius muscle under the influence of Walker-tumour development and nutritional supplementation with leucine. The minimum number of animals per group was 6. The animals were euthanised at different times during tumour development, including the 7th, 14th, and 21st days of the experiment. Legend: C, control group; W, Walker tumour-bearing group; L, rats fed a leucine-rich diet; WL, tumour-bearing rats fed a leucine-rich diet. Graphics represent mean ± standard deviation. Statistical significance is presented in Table 1 after two-way Anova analysis, followed by the Fisher’s LSD multiple comparison test; P < 0.05