Fig. 2
a. The Sangar sequencing results around rs2125739 of the genomic DNA samples from HeLa parent cells (T/T variant in rs2125739), HeLa CRISPR1 cells (T/C) and CRISPR2 cells (C/C). b. The differences in cytotoxicity to docetaxel between the parent and CRISPR-based genome-edited cells. HeLa CRISPR1 cells (T/C) and CRISPR2 cells (C/C) had significantly higher sensitivity to 100 μM docetaxel than did the HeLa parent cells (T/T). Similarly, HeLa parent cells (T/T) had a higher IC50 compared to HeLa CRISPR1 cells (T/C) and CRISPR2 cells (C/C) (T/T vs. T/C vs. C/C; 2.558 nM (95% CI = 2.051–3.192) vs. 1.246 nM (95% CI = 0.8581–1.810) vs. 0.8546 nM (95% CI = 0.6897–1.059), respectively). In this assay, 20 μM verapamil was added to the medium. Each bar represents the mean ± SD of three independent measurements. *P < 0.001. c. The protein expression of ABCC10/MRP7 was the same in HeLa parent cells (T/T), HeLa CRISPR1 cells (T/C) and CRISPR2 cells (C/C) as determined by western blotting