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Fig. 2 | BMC Cancer

Fig. 2

From: Molecular iodine exerts antineoplastic effects by diminishing proliferation and invasive potential and activating the immune response in mammary cancer xenografts

Fig. 2

Effect of I2 supplement on tumoral incidence, tumoral volume and body weight gain in animals with xenografts of cancer cells. Female athymic homozygous (Foxn1 nu/nu) mice were inoculated with 5 × 106 cells of each cell line in 50 μl PBS and 50 μl Matrigel. The drinking water and the water used for 0.025% I2 solution were always deionized. The water supplement (alone or with I2) began 48 h after cell inoculation and was maintained throughout the study. Parameters were analyzed after 6 weeks of inoculations. a, Tumoral incidence. Number of animals that presented observable tumorous mass (0.2 cm3). A second group of animals with 10 animals was incorporated to obtain five samples of xenografts from the MDA-MB231+ I2 group. * Significant differences between I2-treated mice and control mice using Chi-square test. b, Final tumoral volume. Each dot represents an individual tumor by each group. Data are expressed ± SD. * Significant difference (Student’s t-test; P < 0.05) between their respective control. Photographs of representative tumor mass for each group. c, Body weight gain. Lines represent the body weight gain in animals implanted with xenografts and supplemented or not with 0.025% I2 for 6 weeks. The graph also shows the weight recorded for homo and heterozygous non-implanted animals (with and without I2 supplement) at 0, 3 and 6 weeks. Data are expressed ± SD. * Significant difference, one-way ANOVA and Tukey’s test. d, Correlation analysis. Linear regression between final tumor volume (cm3) and final body weight (6th week) in implanted homozygous mice with and without I2 supplement. P value for MCF-7, 0.6661; MCF-7 + I2, 0.9629; MDA-MB231, 0.0852; MDA-MB231 + I2, 0.2865. No significant differences were found

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