Table 1 The primers and probes sequences and PCR product length
Gene name | Primer and probe sequence | Primer and probe length | Product length |
|---|---|---|---|
HPRT1 | F: AGCCTAAGATGAGAGTTC | 18 | 88 |
R: CACAGAACTAGAACATTGATA | 21 | ||
FAM -CATCTGGAGTCCTATTGACATCGC- TAMRA | 24 | ||
NEAT1 | F: CCAGTGTGAGTCCTAGCATTGC | 20 | 78 |
R: CCTGGAAACAGAACATTGGAGAAC | 22 | ||
FAM- ACCCTGGAGGAGAGAGCCCGCC - TAMRA | 23 | ||
TUG1 | F: ACCGGAGGAGCCATCTTGTC | 24 | 149 |
R: GAAAGAGCCGCCAACCGATC | 24 | ||
FAM - ACCGCACGCCCGTTCCTTCGC -TAMRA | 24 | ||
FAS-AS1 | F: GAAAAGGTGCCGTTCTTCCG | 20 | 81 |
R: CTGGCAGTTCTCAGACGTAGG | 20 | ||
FAM - CGGCTTAACCACTGCTTCGGTGCT -TAMRA | 23 | ||
GAS5 | F: CTGCTTGAAAGGGTCTTGCC | 23 | 91 |
R: GGAGGCTGAGGATCACTTGAG | 23 | ||
FAM- ACCCAAGCTAGAGTGCAGTGGCCT- TAMRA | 24 | ||
PVT1 | F: CCCATTACGATTTCATCTC | 20 | 131 |
R: GTTCGTACTCATCTTATTCAA | 21 | ||
FAM- AGCAAGCACCTGTTACCTGTC - TAMRA | 20 | ||
HOTAIRM1 | F: GAAGAGCAAAAGCTGCGTTCTG | 22 | 135 |
R: CTCTCGCCAGTTCATCTTTCATTG | 24 | ||
FAM-CCCGACTCCGCTGCCCGCCC-TAMRA | 20 | ||
THRIL | F: GAGTGCAGTGGCGTGATCTC | 20 | 121 |
R: AAAATTAGTCAGGCATGGTGGTG | 20 | ||
FAM- CTCACCGCAACCTCCACCTCCCAG- TAMRA | 23 |