Fig. 4

Lidocaine induced inhibition of growth and metastasis of MKN45 cells were attenuated by miR-145 silence. a miR-145 expression was decreased after transfection with miR-145 inhibitor. miR-145 knockdown (b) increased cell proliferation, (c) up-regulated Cyclin D1 expression and down-regulated p21 expression, (d) inhibited apoptosis, (e) increased Bcl-2 expression, decreased cleaved-Caspase-3, -7, and -9 expression. miR-145 knockdown (f) increased migration, (g) up-regulated MMP-2 and MMP-9 expression, (h) increased invasion, and (i) up-regulated Vimentin. *p < 0.05, ** p < 0.01, *** p < 0.001