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Fig. 5 | BMC Cancer

Fig. 5

From: RETRACTED ARTICLE: 8-bromo-7-methoxychrysin suppress stemness of SMMC-7721 cells induced by co-culture of liver cancer stem-like cells with hepatic stellate cells

Fig. 5

Addition of HGF attenuated inhibitory effect of BrMC and chrysin on stemness of SMMC-7721 cells. a The representative images of sphere forming cells from SMMC-7721 cells incubated with LCSLC-CM added HGF treated with chryin or BrMC(left). Sphere forming rate was determined by sphere formation assay (* P < 0.05 vs incubated with LCSLC-CM added HGF; # P < 0.05 vs incubated with LCSLC-CM added HGF treated with chryin or BrMC) (right). b Western blot analyzed the expressions of CD133 and CD44 in SMMC-7721 cells incubated with LCSLC-CM added HGF treated with chryin or BrMC. β-actin was used as loading control (left).Densitometry analysis quantify the expression of CD133 and CD44 (* P < 0.05 vs incubated with LCSLC-CM added HGF; # P < 0.05 vs incubated with LCSLC-CM added HGF treated with chryin or BrMC) (right).c Western blot analyzed the expressions of FAP-α. β-actin was used as a loading control (left). Densitometry analysis quantify the expression of FAP-α. (* P < 0.05 vs incubated with LCSLC-CM added HGF; # P < 0.05 vs incubated with LCSLC-CM added HGF treated with chryin or BrMC) (right)

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BMC Cancer

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