Fig. 1

Effects of CX-4945 on CK2 activity and PI3K/AKT pathway phosphorylation in B-ALL cell lines. a CK2 activity in SEM and RS4;11 cells was assessed by western blot analysis of phosphorylated CK2 target sites following 2 h (SEM) or 24 h (RS4;11) incubation with CX-4945 (5 μM) (lower panel). For quantification Image Studio Lite software (Version 5.2) was used to determine signal intensities of phosphorylated CK2 target sites and total CK2 alpha 1 protein. Protein bands were normalized to the GAPDH band of the respective sample and the ratio of phosphorylated targets to total CK2A1 protein signal intensities was calculated. Means and SD of three independent experiments are depicted as bar charts in the upper panel. Individual ratios of one representative blot are stated below the respective bands. b Activity of PTEN, AKT and 4EBP1 proteins was analyzed by western blot of phosphorylated and total protein forms after 2 h (SEM) or 24 h (RS4;11) incubation with 5 μM CX-4945 (mean + SD; n = 3)