Table 2 Primers used for real-time PCR
Primers | Forward | Reverse |
|---|---|---|
β2M | 5′-CGGGCATTCCTGAAGCTGA-3’ | 5′-GGATGGATGAAACCCAGACACATAG-3’ |
HIF-1α | 5′-GAAGTGTACCCTAACTAGCCGAGGA-3’ | 5′-TGAATGTGGCCTGTGCAGTG-3’ |
VEGF | 5′-TGCCATCCAATCGAGACCCTG-3’ | 5′-GGTGATGTTGGACTCCTCAGTG-3’ |
Bcl-2 | 5′-CCTGTGGATGACTGAGTACCTGAAC-3’ | 5′-CAGAGTCTTCAGAGACAGCCAGGA-3’ |
HER2 | 5′-GACGCCTGATGGGTTAATGAG-3’ | 5′-GTGCTGGAGGTAGAGTGGTGAA-3’ |
GAPDH | 5′-GCACCGTCAAGGCTGAGAAC-3’ | 5′-TGGTGAAGACGCCAGTGGA-3’ |