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Fig. 2 | BMC Cancer

Fig. 2

From: β2-microglobulin has a different regulatory molecular mechanism between ER+ and ER− breast cancer with HER2−

Fig. 2

Effects of β2M overexpression in two types of HER2− breast cancer cell lines. pEGFP-C1 (control) or pEGFP-C1-β2M (β2M overexpression group) plasmids were transfected into cells (MCF-7 (a), T47D (b), MDA-MB-231 (c), and Hs578T (d)) for 36 h. Total RNAs were extracted, and the mRNA levels mentioned above were evaluated using real-time PCR. The relative mRNA levels were normalized to that of GAPDH and shown as a histogram (left of panels a-d). Meanwhile, whole cell lysates were prepared from cells and analyzed by western blotting for the indicated proteins. Representative immunoblots are shown in the middle of panels a-d. β-actin was used as loading control. Relative protein signal intensities were quantitatively analyzed using Image Lab software and shown as histograms (right of panels a-d). Values are presented as the mean ± SD; * p < 0.05, ** p < 0.01 compared to the control group. N.S. indicates a non-specific band

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BMC Cancer

ISSN: 1471-2407

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