Fig. 3
From: Isocytosine deaminase Vcz as a novel tool for the prodrug cancer therapy
The cytotoxicity test of 5-FIC methabolic product excreted by Vcz expressing cells. a U87 MG and b Caco-2 cells were transiently transfected with Vcz-encoding vector pTO/Vcz-IG or control vector pcDNA4/TO (designated as pTO) and subsequently treated with 100 μM 5-FIC or DMSO for the additional 72 h. The calculations of Relative Absorbance values of treatment groups were carried out in the same way as for the Fig. 1. c U87 MG and Vcz stably-expressing cells U87Vcz-IG were treated the same way as transfected cells in a and b. Statistical significance indicated by p-values, where the asterisk symbol (*) designates p < 0.0001 with respect to control (DMSO)