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Fig. 2 | BMC Cancer

Fig. 2

From: Isocytosine deaminase Vcz as a novel tool for the prodrug cancer therapy

Fig. 2

Expression of Vcz in the mammalian cells. a Schematic presentation of the pTO/Vcz-IG expression vector encoding, as indicated, the FLAG- (designated as F) tagged Vcz, IRES2 element (designated as IRES) and EGFP. b Expression of FLAG-tagged Vcz in U87MG cells transfected with the empty vector pcDNA4/TO (control, lane 1) and pTO/Vcz-IG (lane 2) and in the stable cell line U87Vcz-IG (lane 3) was detected by Western blot analysis, using FLAG-specific antibodies. The positions of the molecular mass standards (in kDa) and FLAG-Vcz protein (Vcz) are indicated. Expression of EGFP in c U87MG cells transfected with pTO/Vcz-IG and d in the stable cell line U87Vcz-IG were analyzed by fluorescence microscopy (left panels). Bright field views of the cells are presented in right panels

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