Fig. 3

miR-148b inhibited the proliferation of NSCLC cells. The total number of viable PC14/B a and A549 cells b after being transfected with different mimics was assessed using a Cell Counting Kit-8 assay. The data are expressed as optical density (OD). c and d The colony formation assay for PC14/B and A549 cells. Transfected with different mimics. Over-expression of miR-148b significantly inhibited PC14/B and A549 cell proliferation and colony formation compared to the blank group. While knocking down miR-148b expression (NC) could reverse miR-148b-mediated proliferation and colony formation inhibiting effect in PC14/B and A549 cells. The results are expressed as the mean ± standard deviation. NC, negative control; miR, microRNA; Blank, the cells transfected with unpackaged lentiviral vectors; OD, optical density