Fig. 5

Ajoene Inhibits the Invasion and Migration of MDA-MB-231 and HeLa Cells. Wound healing assay: Following introduction of a scratch wound into the cell layer of (a and c) MDA-MB-231 or (b and d) HeLa cells; native cells, or cells silenced for vimentin expression were incubated with 10 μM ZA in DMSO or DMSO alone for 24 h. Migration into the wound was then quantified using Image J software. Transwell invasion and migration assay: (e) MDA-MB-231 cells or (f) HeLa cells were transfected with vimentin siRNA and treated as described above for 24 h. The ability of cells to invade and migrate through the matrigel membrane was quantified by counting the crystal violet stained cells, data displayed as mean ± SD. The results of a single representative experiment is shown; however experiments were performed in duplicate