Fig. 3

Ajoene Disrupts the Vimentin Filament Network in MDA-MB-231 and HeLa cells. 40x Phase contrast images of MDA-MB-231 (a) or HeLa (d) cells treated with DMSO (control) or 10 μM ZA in DMSO for 24 h. Cell viability assay: MDA-MB-231 (b) or HeLa (e) cells treated with DMSO (control) or with ZA (0, 5, 10, 20 or 40 μM) for 6 h or 24 h. Immunofluorescence: MDA-MB-231 (c) or HeLa (f) cells treated with 20 μM ZA for 6 h, then fixed and immunostained with vimentin primary antibodies (V9, H84 or V4630). Control cells treated with DMSO alone. Images obtained by confocal scanning laser microscopy