Fig. 1

Purification and Identification of Vimentin from DP-treated MDA-MB-231 cells. Proposed disulfide exchange reaction occurring between a cysteine sulfhydryl group on a target protein with (a) Z-ajoene (ZA) or its analogue (b) dansyl-ajoene (DP). (c) Lysate collected from MDA-MB-231 breast cancer cells treated with 25 μM ZA or DP show many dansyl-labelled proteins by immunoblot when probed with an anti-dansyl primary antibody in the DP-treated sample only. The experiment was performed under non-reducing conditions. (d) Separation of the dansyl-labelled proteins in MDA-MB-231 cell lysate by 2D gel electrophoresis under non-reducing conditions. A predominant band (circled) was observed in the immunoblot which was excised from the corresponding gel and identified by MALDI-TOF MS/MS to be vimentin