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Fig. 2 | BMC Cancer

Fig. 2

From: Circulating tumor cell investigation in breast cancer patient-derived xenograft models by automated immunofluorescence staining, image acquisition, and single cell retrieval and analysis

Fig. 2

Comparison of modified RareCyte method to IHC method in detecting CTCs in BC-PDX-bearing mice. a. Representative immunofluorescence images of CTCs. Images show morphology of recovered CTCs. Column 1 is a composite/merged image; column 2 is DAPI staining of nuclei; column 3 is human cytokeratin staining; column 4 is mouse CD45 staining; and column 5 is staining with a cocktail of EpCAM, EGFR and HER2. (Scale bar equals approximately 20 μm) b. Comparison of IHC and RareCyte method for detecting CTCs in PDX models BCM-4272 and BCM-3887, which was not significantly different between IHC and RareCyte methods (N = 4; P ≥ 0.05, Paired t-test). c. Collective expression of cell surface markers (EpCAM, EGFR, HER2) on CTCs and BC-PDX tumor cells collected from mammary tumor and lung metastatic nodules in BCM-4888 model

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BMC Cancer

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